p erk (Cell Signaling Technology Inc)
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P Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 13425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 13425 article reviews
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Blocking Assay:Article Title: Euonymus alatus Leaf Extract Attenuates Effects of Aging on Oxidative Stress, Neuroinflammation, and Cognitive Impairment Article Snippet: The primary antibody against BDNF (bsm-52368R) was obtained from Bioss (Beijing, China), whereas antibodies against p-p65NF-κB (CST3031), p65NF-κB (CST3034), p-ERK (CST9102), ERK (CST9102), p-CREB (CST9198), and Article Title: Novel long noncoding lncARF mediated hyperhomocysteinemia-induced atherosclerosis via autophagy inhibition in foam cells. Article Snippet: After blocking with 5 % non-fat milk, the membranes were incubated overnight at 4 C with specific primary antibodies: LC3B (1:1000, Abcam, Cambridge, USA), p62 (1:1000, Abcam, Cambridge, USA), RRAGD (1:1000, Abmart, Shanghai, China), ALIX (1:1000, Abcam, Cambridge, USA), ERP29 (1:1000, Abcam, Cambridge, USA), Ubiquitin (1:1000, Abcam, Cambridge, USA), Article Title: Pharmacological modulation of gp130 signalling enhances Achilles tendon repair by regulating tenocyte migration and collagen synthesis via SHP2-mediated crosstalk of the ERK/AKT pathway. Article Snippet: Tendon injuries typically display limited reparative capacity, often resulting in suboptimal outcomes and an elevated risk of recurrence or rupture.. While cytokines of the IL-6 family are primarily recognised for their inflammatory properties, they also have multifaceted roles in tissue regeneration and repair.. Despite this, studies examining the association between IL-6 family cytokines and tendon repair remained scarce. gp130, a type of glycoprotein, functions as a co-receptor for all cytokines in the IL-6 family. Article Title: Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress Article Snippet: The following antibodies were used: Synapsin-I (SYN, 1:4000, Abclone), Synaptophysin (SYP, 1:4000, Proteintech), Brain-derived neurotrophic factor (BDNF, 1:2000, Abcam), Postsynaptic density protein 95 (PSD95, 1:4000, Proteintech), β-Tubulin (1:10000, HUABIO), Article Title: Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress. Article Snippet: Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry.. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects.. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the antiviral qualities of BBR against SGIV. Article Title: Autophagy inhibition potentiates energy restriction-induced cell death in hepatocellular carcinoma cells. Article Snippet: Funding information University of Sharjah, Grant/Award Number: 2201110357 Abstract Hepatocellular carcinoma (HCC) significantly contributes to cancer-related mortality due to the limited response of HCC to current anticancer therapies, thereby necessitating more effective treatment approaches.. Energy restriction mimetic agents (ERMAs) have emerged as potential therapies in targeting the Warburg effect, a unique metabolic process in cancer cells.. However, ERMAs exhibit limited efficacy when used as monotherapy. Article Title: Neuroprotective effects of the salidroside derivative SHPL-49 via the BDNF/TrkB/Gap43 pathway in rats with cerebral ischemia. Article Snippet: Membranes were incubated with 5% nonfat milk (Beyotime) or BSA (Beyotime) followed by overnight incubation at 4 ◦C with the following primary antibodies: BDNF (1:1000, Abcam), TrkB (1:1000, Abclonal), Article Title: Marine derivative CHNQD-00603 reverses bone marrow mesenchymal stem cells senescence by enhancing autophagy through the AKT/ERK/mTOR signaling pathway Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with the primary antibodies GAPDH(1:2000; Elabscience, cat. no. E-AB-20059, China), P62 (1:1000; Catalog No.AF5484; A nity Biosciences), LC3(1:1000; Proteintech, cat. no. 16400-1-AP, China), Incubation:Article Title: Euonymus alatus Leaf Extract Attenuates Effects of Aging on Oxidative Stress, Neuroinflammation, and Cognitive Impairment Article Snippet: The primary antibody against BDNF (bsm-52368R) was obtained from Bioss (Beijing, China), whereas antibodies against p-p65NF-κB (CST3031), p65NF-κB (CST3034), p-ERK (CST9102), ERK (CST9102), p-CREB (CST9198), and Article Title: Novel long noncoding lncARF mediated hyperhomocysteinemia-induced atherosclerosis via autophagy inhibition in foam cells. Article Snippet: After blocking with 5 % non-fat milk, the membranes were incubated overnight at 4 C with specific primary antibodies: LC3B (1:1000, Abcam, Cambridge, USA), p62 (1:1000, Abcam, Cambridge, USA), RRAGD (1:1000, Abmart, Shanghai, China), ALIX (1:1000, Abcam, Cambridge, USA), ERP29 (1:1000, Abcam, Cambridge, USA), Ubiquitin (1:1000, Abcam, Cambridge, USA), Article Title: Pharmacological modulation of gp130 signalling enhances Achilles tendon repair by regulating tenocyte migration and collagen synthesis via SHP2-mediated crosstalk of the ERK/AKT pathway. Article Snippet: Tendon injuries typically display limited reparative capacity, often resulting in suboptimal outcomes and an elevated risk of recurrence or rupture.. While cytokines of the IL-6 family are primarily recognised for their inflammatory properties, they also have multifaceted roles in tissue regeneration and repair.. Despite this, studies examining the association between IL-6 family cytokines and tendon repair remained scarce. gp130, a type of glycoprotein, functions as a co-receptor for all cytokines in the IL-6 family. Article Title: Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress Article Snippet: The following antibodies were used: Synapsin-I (SYN, 1:4000, Abclone), Synaptophysin (SYP, 1:4000, Proteintech), Brain-derived neurotrophic factor (BDNF, 1:2000, Abcam), Postsynaptic density protein 95 (PSD95, 1:4000, Proteintech), β-Tubulin (1:10000, HUABIO), Article Title: Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress. Article Snippet: Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry.. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects.. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the antiviral qualities of BBR against SGIV. Article Title: Autophagy inhibition potentiates energy restriction-induced cell death in hepatocellular carcinoma cells. Article Snippet: Funding information University of Sharjah, Grant/Award Number: 2201110357 Abstract Hepatocellular carcinoma (HCC) significantly contributes to cancer-related mortality due to the limited response of HCC to current anticancer therapies, thereby necessitating more effective treatment approaches.. Energy restriction mimetic agents (ERMAs) have emerged as potential therapies in targeting the Warburg effect, a unique metabolic process in cancer cells.. However, ERMAs exhibit limited efficacy when used as monotherapy. Article Title: Neuroprotective effects of the salidroside derivative SHPL-49 via the BDNF/TrkB/Gap43 pathway in rats with cerebral ischemia. Article Snippet: Membranes were incubated with 5% nonfat milk (Beyotime) or BSA (Beyotime) followed by overnight incubation at 4 ◦C with the following primary antibodies: BDNF (1:1000, Abcam), TrkB (1:1000, Abclonal), Article Title: Marine derivative CHNQD-00603 reverses bone marrow mesenchymal stem cells senescence by enhancing autophagy through the AKT/ERK/mTOR signaling pathway Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with the primary antibodies GAPDH(1:2000; Elabscience, cat. no. E-AB-20059, China), P62 (1:1000; Catalog No.AF5484; A nity Biosciences), LC3(1:1000; Proteintech, cat. no. 16400-1-AP, China), Western Blot:Article Title: Euonymus alatus Leaf Extract Attenuates Effects of Aging on Oxidative Stress, Neuroinflammation, and Cognitive Impairment Article Snippet: The primary antibody against BDNF (bsm-52368R) was obtained from Bioss (Beijing, China), whereas antibodies against p-p65NF-κB (CST3031), p65NF-κB (CST3034), p-ERK (CST9102), ERK (CST9102), p-CREB (CST9198), and Article Title: Novel long noncoding lncARF mediated hyperhomocysteinemia-induced atherosclerosis via autophagy inhibition in foam cells. Article Snippet: After blocking with 5 % non-fat milk, the membranes were incubated overnight at 4 C with specific primary antibodies: LC3B (1:1000, Abcam, Cambridge, USA), p62 (1:1000, Abcam, Cambridge, USA), RRAGD (1:1000, Abmart, Shanghai, China), ALIX (1:1000, Abcam, Cambridge, USA), ERP29 (1:1000, Abcam, Cambridge, USA), Ubiquitin (1:1000, Abcam, Cambridge, USA), Article Title: Pharmacological modulation of gp130 signalling enhances Achilles tendon repair by regulating tenocyte migration and collagen synthesis via SHP2-mediated crosstalk of the ERK/AKT pathway. Article Snippet: Tendon injuries typically display limited reparative capacity, often resulting in suboptimal outcomes and an elevated risk of recurrence or rupture.. While cytokines of the IL-6 family are primarily recognised for their inflammatory properties, they also have multifaceted roles in tissue regeneration and repair.. Despite this, studies examining the association between IL-6 family cytokines and tendon repair remained scarce. gp130, a type of glycoprotein, functions as a co-receptor for all cytokines in the IL-6 family. Article Title: Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress Article Snippet: The following antibodies were used: Synapsin-I (SYN, 1:4000, Abclone), Synaptophysin (SYP, 1:4000, Proteintech), Brain-derived neurotrophic factor (BDNF, 1:2000, Abcam), Postsynaptic density protein 95 (PSD95, 1:4000, Proteintech), β-Tubulin (1:10000, HUABIO), Article Title: Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress. Article Snippet: Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry.. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects.. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the antiviral qualities of BBR against SGIV. Article Title: Autophagy inhibition potentiates energy restriction-induced cell death in hepatocellular carcinoma cells. Article Snippet: Funding information University of Sharjah, Grant/Award Number: 2201110357 Abstract Hepatocellular carcinoma (HCC) significantly contributes to cancer-related mortality due to the limited response of HCC to current anticancer therapies, thereby necessitating more effective treatment approaches.. Energy restriction mimetic agents (ERMAs) have emerged as potential therapies in targeting the Warburg effect, a unique metabolic process in cancer cells.. However, ERMAs exhibit limited efficacy when used as monotherapy. Article Title: Neuroprotective effects of the salidroside derivative SHPL-49 via the BDNF/TrkB/Gap43 pathway in rats with cerebral ischemia. Article Snippet: Membranes were incubated with 5% nonfat milk (Beyotime) or BSA (Beyotime) followed by overnight incubation at 4 ◦C with the following primary antibodies: BDNF (1:1000, Abcam), TrkB (1:1000, Abclonal), Article Title: Marine derivative CHNQD-00603 reverses bone marrow mesenchymal stem cells senescence by enhancing autophagy through the AKT/ERK/mTOR signaling pathway Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with the primary antibodies GAPDH(1:2000; Elabscience, cat. no. E-AB-20059, China), P62 (1:1000; Catalog No.AF5484; A nity Biosciences), LC3(1:1000; Proteintech, cat. no. 16400-1-AP, China), Expressing:Article Title: Euonymus alatus Leaf Extract Attenuates Effects of Aging on Oxidative Stress, Neuroinflammation, and Cognitive Impairment Article Snippet: The primary antibody against BDNF (bsm-52368R) was obtained from Bioss (Beijing, China), whereas antibodies against p-p65NF-κB (CST3031), p65NF-κB (CST3034), p-ERK (CST9102), ERK (CST9102), p-CREB (CST9198), and Article Title: Novel long noncoding lncARF mediated hyperhomocysteinemia-induced atherosclerosis via autophagy inhibition in foam cells. Article Snippet: After blocking with 5 % non-fat milk, the membranes were incubated overnight at 4 C with specific primary antibodies: LC3B (1:1000, Abcam, Cambridge, USA), p62 (1:1000, Abcam, Cambridge, USA), RRAGD (1:1000, Abmart, Shanghai, China), ALIX (1:1000, Abcam, Cambridge, USA), ERP29 (1:1000, Abcam, Cambridge, USA), Ubiquitin (1:1000, Abcam, Cambridge, USA), Article Title: Pharmacological modulation of gp130 signalling enhances Achilles tendon repair by regulating tenocyte migration and collagen synthesis via SHP2-mediated crosstalk of the ERK/AKT pathway. Article Snippet: Tendon injuries typically display limited reparative capacity, often resulting in suboptimal outcomes and an elevated risk of recurrence or rupture.. While cytokines of the IL-6 family are primarily recognised for their inflammatory properties, they also have multifaceted roles in tissue regeneration and repair.. Despite this, studies examining the association between IL-6 family cytokines and tendon repair remained scarce. gp130, a type of glycoprotein, functions as a co-receptor for all cytokines in the IL-6 family. Article Title: Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress Article Snippet: The following antibodies were used: Synapsin-I (SYN, 1:4000, Abclone), Synaptophysin (SYP, 1:4000, Proteintech), Brain-derived neurotrophic factor (BDNF, 1:2000, Abcam), Postsynaptic density protein 95 (PSD95, 1:4000, Proteintech), β-Tubulin (1:10000, HUABIO), Article Title: Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress. Article Snippet: Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry.. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects.. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the antiviral qualities of BBR against SGIV. Article Title: Autophagy inhibition potentiates energy restriction-induced cell death in hepatocellular carcinoma cells. Article Snippet: Funding information University of Sharjah, Grant/Award Number: 2201110357 Abstract Hepatocellular carcinoma (HCC) significantly contributes to cancer-related mortality due to the limited response of HCC to current anticancer therapies, thereby necessitating more effective treatment approaches.. Energy restriction mimetic agents (ERMAs) have emerged as potential therapies in targeting the Warburg effect, a unique metabolic process in cancer cells.. However, ERMAs exhibit limited efficacy when used as monotherapy. Article Title: Neuroprotective effects of the salidroside derivative SHPL-49 via the BDNF/TrkB/Gap43 pathway in rats with cerebral ischemia. Article Snippet: Membranes were incubated with 5% nonfat milk (Beyotime) or BSA (Beyotime) followed by overnight incubation at 4 ◦C with the following primary antibodies: BDNF (1:1000, Abcam), TrkB (1:1000, Abclonal), Article Title: Marine derivative CHNQD-00603 reverses bone marrow mesenchymal stem cells senescence by enhancing autophagy through the AKT/ERK/mTOR signaling pathway Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with the primary antibodies GAPDH(1:2000; Elabscience, cat. no. E-AB-20059, China), P62 (1:1000; Catalog No.AF5484; A nity Biosciences), LC3(1:1000; Proteintech, cat. no. 16400-1-AP, China), |
