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    Cell Signaling Technology Inc p erk
    P Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 13425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-erk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+XP+Rabbit+mAb/pmc13042271-96-58-63
    Average 99 stars, based on 13425 article reviews
    p erk - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Euonymus alatus Leaf Extract Attenuates Effects of Aging on Oxidative Stress, Neuroinflammation, and Cognitive Impairment
    Article Snippet: The primary antibody against BDNF (bsm-52368R) was obtained from Bioss (Beijing, China), whereas antibodies against p-p65NF-κB (CST3031), p65NF-κB (CST3034), p-ERK (CST9102), ERK (CST9102), p-CREB (CST9198), and CREB (CST4820) were procured from Cell Signaling Technology Inc. (Beverly, MA, USA).

    Article Title: Novel long noncoding lncARF mediated hyperhomocysteinemia-induced atherosclerosis via autophagy inhibition in foam cells.
    Article Snippet: After blocking with 5 % non-fat milk, the membranes were incubated overnight at 4 C with specific primary antibodies: LC3B (1:1000, Abcam, Cambridge, USA), p62 (1:1000, Abcam, Cambridge, USA), RRAGD (1:1000, Abmart, Shanghai, China), ALIX (1:1000, Abcam, Cambridge, USA), ERP29 (1:1000, Abcam, Cambridge, USA), Ubiquitin (1:1000, Abcam, Cambridge, USA), pMAPK (1:1000, Cell Signaling Technology, Boston, USA), MAPK (1:1000, Cell Signaling Technology, Boston, USA), p-ERK (1:1000, Cell Signaling Technology, Boston, USA), ERK (1:1000, Cell Signaling Technology, Boston, USA), p-PI3K (1:1000, Cell Signaling Technology, Boston, USA), PI3K (1:1000, Cell Signaling Technology, Boston, USA), p-Akt (1:1000, Cell Signaling Technology, Boston, USA), Akt (1:1000, Cell Signaling Technology, Boston, USA), FosB (1:1000, Abcam, Cambridge, USA), DNMT1 (1:1000, Abcam, Cambridge, USA), and b-actin (1:1000, Zhongshan Biotech, Beijing, China).

    Article Title: Pharmacological modulation of gp130 signalling enhances Achilles tendon repair by regulating tenocyte migration and collagen synthesis via SHP2-mediated crosstalk of the ERK/AKT pathway.
    Article Snippet: Tendon injuries typically display limited reparative capacity, often resulting in suboptimal outcomes and an elevated risk of recurrence or rupture.. While cytokines of the IL-6 family are primarily recognised for their inflammatory properties, they also have multifaceted roles in tissue regeneration and repair.. Despite this, studies examining the association between IL-6 family cytokines and tendon repair remained scarce. gp130, a type of glycoprotein, functions as a co-receptor for all cytokines in the IL-6 family.

    Article Title: Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress
    Article Snippet: The following antibodies were used: Synapsin-I (SYN, 1:4000, Abclone), Synaptophysin (SYP, 1:4000, Proteintech), Brain-derived neurotrophic factor (BDNF, 1:2000, Abcam), Postsynaptic density protein 95 (PSD95, 1:4000, Proteintech), β-Tubulin (1:10000, HUABIO), p-ERK (1:1000, Cell Signaling Technology), ERK (1:2000, Cell Signaling Technology), p-CREB (1:1000, Cell Signaling Technology), and CREB (1:2000, Cell Signaling Technology).

    Article Title: Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress.
    Article Snippet: Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry.. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects.. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the antiviral qualities of BBR against SGIV.

    Article Title: Autophagy inhibition potentiates energy restriction-induced cell death in hepatocellular carcinoma cells.
    Article Snippet: Funding information University of Sharjah, Grant/Award Number: 2201110357 Abstract Hepatocellular carcinoma (HCC) significantly contributes to cancer-related mortality due to the limited response of HCC to current anticancer therapies, thereby necessitating more effective treatment approaches.. Energy restriction mimetic agents (ERMAs) have emerged as potential therapies in targeting the Warburg effect, a unique metabolic process in cancer cells.. However, ERMAs exhibit limited efficacy when used as monotherapy.

    Article Title: Neuroprotective effects of the salidroside derivative SHPL-49 via the BDNF/TrkB/Gap43 pathway in rats with cerebral ischemia.
    Article Snippet: Membranes were incubated with 5% nonfat milk (Beyotime) or BSA (Beyotime) followed by overnight incubation at 4 ◦C with the following primary antibodies: BDNF (1:1000, Abcam), TrkB (1:1000, Abclonal), p-AKT (1:1000, Cell Signaling Technology), AKT (1:1000, Cell Signaling Technology), p-ERK (1:1000, Cell Signaling Technology), ERK (1:1000, Cell Signaling Technology), or growthassociated protein 43 (Gap43) (1:200, Santa Cruz).

    Article Title: Marine derivative CHNQD-00603 reverses bone marrow mesenchymal stem cells senescence by enhancing autophagy through the AKT/ERK/mTOR signaling pathway
    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with the primary antibodies GAPDH(1:2000; Elabscience, cat. no. E-AB-20059, China), P62 (1:1000; Catalog No.AF5484; A nity Biosciences), LC3(1:1000; Proteintech, cat. no. 16400-1-AP, China), AKT (1:1000; Cell Signaling Technology, cat. no.C67E7 China), P-AKT(1:1000; Cell Signaling Technology, cat. no.D25E6 China), ERK (1:1000; Cell Signaling Technology, cat. no.137F5 China), P-ERK(1:2000; Cell Signaling Technology, cat. no.D13.14.4E China), overnight at 4 ◦ C and then incubated for 1h at 37°C with secondary antibodies, anti-Rabbit IgG (1:10000, 1:10,000; Proteintech, cat. no. 10285-1-AP).

    Incubation:

    Article Title: Euonymus alatus Leaf Extract Attenuates Effects of Aging on Oxidative Stress, Neuroinflammation, and Cognitive Impairment
    Article Snippet: The primary antibody against BDNF (bsm-52368R) was obtained from Bioss (Beijing, China), whereas antibodies against p-p65NF-κB (CST3031), p65NF-κB (CST3034), p-ERK (CST9102), ERK (CST9102), p-CREB (CST9198), and CREB (CST4820) were procured from Cell Signaling Technology Inc. (Beverly, MA, USA).

    Article Title: Novel long noncoding lncARF mediated hyperhomocysteinemia-induced atherosclerosis via autophagy inhibition in foam cells.
    Article Snippet: After blocking with 5 % non-fat milk, the membranes were incubated overnight at 4 C with specific primary antibodies: LC3B (1:1000, Abcam, Cambridge, USA), p62 (1:1000, Abcam, Cambridge, USA), RRAGD (1:1000, Abmart, Shanghai, China), ALIX (1:1000, Abcam, Cambridge, USA), ERP29 (1:1000, Abcam, Cambridge, USA), Ubiquitin (1:1000, Abcam, Cambridge, USA), pMAPK (1:1000, Cell Signaling Technology, Boston, USA), MAPK (1:1000, Cell Signaling Technology, Boston, USA), p-ERK (1:1000, Cell Signaling Technology, Boston, USA), ERK (1:1000, Cell Signaling Technology, Boston, USA), p-PI3K (1:1000, Cell Signaling Technology, Boston, USA), PI3K (1:1000, Cell Signaling Technology, Boston, USA), p-Akt (1:1000, Cell Signaling Technology, Boston, USA), Akt (1:1000, Cell Signaling Technology, Boston, USA), FosB (1:1000, Abcam, Cambridge, USA), DNMT1 (1:1000, Abcam, Cambridge, USA), and b-actin (1:1000, Zhongshan Biotech, Beijing, China).

    Article Title: Pharmacological modulation of gp130 signalling enhances Achilles tendon repair by regulating tenocyte migration and collagen synthesis via SHP2-mediated crosstalk of the ERK/AKT pathway.
    Article Snippet: Tendon injuries typically display limited reparative capacity, often resulting in suboptimal outcomes and an elevated risk of recurrence or rupture.. While cytokines of the IL-6 family are primarily recognised for their inflammatory properties, they also have multifaceted roles in tissue regeneration and repair.. Despite this, studies examining the association between IL-6 family cytokines and tendon repair remained scarce. gp130, a type of glycoprotein, functions as a co-receptor for all cytokines in the IL-6 family.

    Article Title: Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress
    Article Snippet: The following antibodies were used: Synapsin-I (SYN, 1:4000, Abclone), Synaptophysin (SYP, 1:4000, Proteintech), Brain-derived neurotrophic factor (BDNF, 1:2000, Abcam), Postsynaptic density protein 95 (PSD95, 1:4000, Proteintech), β-Tubulin (1:10000, HUABIO), p-ERK (1:1000, Cell Signaling Technology), ERK (1:2000, Cell Signaling Technology), p-CREB (1:1000, Cell Signaling Technology), and CREB (1:2000, Cell Signaling Technology).

    Article Title: Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress.
    Article Snippet: Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry.. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects.. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the antiviral qualities of BBR against SGIV.

    Article Title: Autophagy inhibition potentiates energy restriction-induced cell death in hepatocellular carcinoma cells.
    Article Snippet: Funding information University of Sharjah, Grant/Award Number: 2201110357 Abstract Hepatocellular carcinoma (HCC) significantly contributes to cancer-related mortality due to the limited response of HCC to current anticancer therapies, thereby necessitating more effective treatment approaches.. Energy restriction mimetic agents (ERMAs) have emerged as potential therapies in targeting the Warburg effect, a unique metabolic process in cancer cells.. However, ERMAs exhibit limited efficacy when used as monotherapy.

    Article Title: Neuroprotective effects of the salidroside derivative SHPL-49 via the BDNF/TrkB/Gap43 pathway in rats with cerebral ischemia.
    Article Snippet: Membranes were incubated with 5% nonfat milk (Beyotime) or BSA (Beyotime) followed by overnight incubation at 4 ◦C with the following primary antibodies: BDNF (1:1000, Abcam), TrkB (1:1000, Abclonal), p-AKT (1:1000, Cell Signaling Technology), AKT (1:1000, Cell Signaling Technology), p-ERK (1:1000, Cell Signaling Technology), ERK (1:1000, Cell Signaling Technology), or growthassociated protein 43 (Gap43) (1:200, Santa Cruz).

    Article Title: Marine derivative CHNQD-00603 reverses bone marrow mesenchymal stem cells senescence by enhancing autophagy through the AKT/ERK/mTOR signaling pathway
    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with the primary antibodies GAPDH(1:2000; Elabscience, cat. no. E-AB-20059, China), P62 (1:1000; Catalog No.AF5484; A nity Biosciences), LC3(1:1000; Proteintech, cat. no. 16400-1-AP, China), AKT (1:1000; Cell Signaling Technology, cat. no.C67E7 China), P-AKT(1:1000; Cell Signaling Technology, cat. no.D25E6 China), ERK (1:1000; Cell Signaling Technology, cat. no.137F5 China), P-ERK(1:2000; Cell Signaling Technology, cat. no.D13.14.4E China), overnight at 4 ◦ C and then incubated for 1h at 37°C with secondary antibodies, anti-Rabbit IgG (1:10000, 1:10,000; Proteintech, cat. no. 10285-1-AP).

    Western Blot:

    Article Title: Euonymus alatus Leaf Extract Attenuates Effects of Aging on Oxidative Stress, Neuroinflammation, and Cognitive Impairment
    Article Snippet: The primary antibody against BDNF (bsm-52368R) was obtained from Bioss (Beijing, China), whereas antibodies against p-p65NF-κB (CST3031), p65NF-κB (CST3034), p-ERK (CST9102), ERK (CST9102), p-CREB (CST9198), and CREB (CST4820) were procured from Cell Signaling Technology Inc. (Beverly, MA, USA).

    Article Title: Novel long noncoding lncARF mediated hyperhomocysteinemia-induced atherosclerosis via autophagy inhibition in foam cells.
    Article Snippet: After blocking with 5 % non-fat milk, the membranes were incubated overnight at 4 C with specific primary antibodies: LC3B (1:1000, Abcam, Cambridge, USA), p62 (1:1000, Abcam, Cambridge, USA), RRAGD (1:1000, Abmart, Shanghai, China), ALIX (1:1000, Abcam, Cambridge, USA), ERP29 (1:1000, Abcam, Cambridge, USA), Ubiquitin (1:1000, Abcam, Cambridge, USA), pMAPK (1:1000, Cell Signaling Technology, Boston, USA), MAPK (1:1000, Cell Signaling Technology, Boston, USA), p-ERK (1:1000, Cell Signaling Technology, Boston, USA), ERK (1:1000, Cell Signaling Technology, Boston, USA), p-PI3K (1:1000, Cell Signaling Technology, Boston, USA), PI3K (1:1000, Cell Signaling Technology, Boston, USA), p-Akt (1:1000, Cell Signaling Technology, Boston, USA), Akt (1:1000, Cell Signaling Technology, Boston, USA), FosB (1:1000, Abcam, Cambridge, USA), DNMT1 (1:1000, Abcam, Cambridge, USA), and b-actin (1:1000, Zhongshan Biotech, Beijing, China).

    Article Title: Pharmacological modulation of gp130 signalling enhances Achilles tendon repair by regulating tenocyte migration and collagen synthesis via SHP2-mediated crosstalk of the ERK/AKT pathway.
    Article Snippet: Tendon injuries typically display limited reparative capacity, often resulting in suboptimal outcomes and an elevated risk of recurrence or rupture.. While cytokines of the IL-6 family are primarily recognised for their inflammatory properties, they also have multifaceted roles in tissue regeneration and repair.. Despite this, studies examining the association between IL-6 family cytokines and tendon repair remained scarce. gp130, a type of glycoprotein, functions as a co-receptor for all cytokines in the IL-6 family.

    Article Title: Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress
    Article Snippet: The following antibodies were used: Synapsin-I (SYN, 1:4000, Abclone), Synaptophysin (SYP, 1:4000, Proteintech), Brain-derived neurotrophic factor (BDNF, 1:2000, Abcam), Postsynaptic density protein 95 (PSD95, 1:4000, Proteintech), β-Tubulin (1:10000, HUABIO), p-ERK (1:1000, Cell Signaling Technology), ERK (1:2000, Cell Signaling Technology), p-CREB (1:1000, Cell Signaling Technology), and CREB (1:2000, Cell Signaling Technology).

    Article Title: Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress.
    Article Snippet: Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry.. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects.. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the antiviral qualities of BBR against SGIV.

    Article Title: Autophagy inhibition potentiates energy restriction-induced cell death in hepatocellular carcinoma cells.
    Article Snippet: Funding information University of Sharjah, Grant/Award Number: 2201110357 Abstract Hepatocellular carcinoma (HCC) significantly contributes to cancer-related mortality due to the limited response of HCC to current anticancer therapies, thereby necessitating more effective treatment approaches.. Energy restriction mimetic agents (ERMAs) have emerged as potential therapies in targeting the Warburg effect, a unique metabolic process in cancer cells.. However, ERMAs exhibit limited efficacy when used as monotherapy.

    Article Title: Neuroprotective effects of the salidroside derivative SHPL-49 via the BDNF/TrkB/Gap43 pathway in rats with cerebral ischemia.
    Article Snippet: Membranes were incubated with 5% nonfat milk (Beyotime) or BSA (Beyotime) followed by overnight incubation at 4 ◦C with the following primary antibodies: BDNF (1:1000, Abcam), TrkB (1:1000, Abclonal), p-AKT (1:1000, Cell Signaling Technology), AKT (1:1000, Cell Signaling Technology), p-ERK (1:1000, Cell Signaling Technology), ERK (1:1000, Cell Signaling Technology), or growthassociated protein 43 (Gap43) (1:200, Santa Cruz).

    Article Title: Marine derivative CHNQD-00603 reverses bone marrow mesenchymal stem cells senescence by enhancing autophagy through the AKT/ERK/mTOR signaling pathway
    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with the primary antibodies GAPDH(1:2000; Elabscience, cat. no. E-AB-20059, China), P62 (1:1000; Catalog No.AF5484; A nity Biosciences), LC3(1:1000; Proteintech, cat. no. 16400-1-AP, China), AKT (1:1000; Cell Signaling Technology, cat. no.C67E7 China), P-AKT(1:1000; Cell Signaling Technology, cat. no.D25E6 China), ERK (1:1000; Cell Signaling Technology, cat. no.137F5 China), P-ERK(1:2000; Cell Signaling Technology, cat. no.D13.14.4E China), overnight at 4 ◦ C and then incubated for 1h at 37°C with secondary antibodies, anti-Rabbit IgG (1:10000, 1:10,000; Proteintech, cat. no. 10285-1-AP).

    Expressing:

    Article Title: Euonymus alatus Leaf Extract Attenuates Effects of Aging on Oxidative Stress, Neuroinflammation, and Cognitive Impairment
    Article Snippet: The primary antibody against BDNF (bsm-52368R) was obtained from Bioss (Beijing, China), whereas antibodies against p-p65NF-κB (CST3031), p65NF-κB (CST3034), p-ERK (CST9102), ERK (CST9102), p-CREB (CST9198), and CREB (CST4820) were procured from Cell Signaling Technology Inc. (Beverly, MA, USA).

    Article Title: Novel long noncoding lncARF mediated hyperhomocysteinemia-induced atherosclerosis via autophagy inhibition in foam cells.
    Article Snippet: After blocking with 5 % non-fat milk, the membranes were incubated overnight at 4 C with specific primary antibodies: LC3B (1:1000, Abcam, Cambridge, USA), p62 (1:1000, Abcam, Cambridge, USA), RRAGD (1:1000, Abmart, Shanghai, China), ALIX (1:1000, Abcam, Cambridge, USA), ERP29 (1:1000, Abcam, Cambridge, USA), Ubiquitin (1:1000, Abcam, Cambridge, USA), pMAPK (1:1000, Cell Signaling Technology, Boston, USA), MAPK (1:1000, Cell Signaling Technology, Boston, USA), p-ERK (1:1000, Cell Signaling Technology, Boston, USA), ERK (1:1000, Cell Signaling Technology, Boston, USA), p-PI3K (1:1000, Cell Signaling Technology, Boston, USA), PI3K (1:1000, Cell Signaling Technology, Boston, USA), p-Akt (1:1000, Cell Signaling Technology, Boston, USA), Akt (1:1000, Cell Signaling Technology, Boston, USA), FosB (1:1000, Abcam, Cambridge, USA), DNMT1 (1:1000, Abcam, Cambridge, USA), and b-actin (1:1000, Zhongshan Biotech, Beijing, China).

    Article Title: Pharmacological modulation of gp130 signalling enhances Achilles tendon repair by regulating tenocyte migration and collagen synthesis via SHP2-mediated crosstalk of the ERK/AKT pathway.
    Article Snippet: Tendon injuries typically display limited reparative capacity, often resulting in suboptimal outcomes and an elevated risk of recurrence or rupture.. While cytokines of the IL-6 family are primarily recognised for their inflammatory properties, they also have multifaceted roles in tissue regeneration and repair.. Despite this, studies examining the association between IL-6 family cytokines and tendon repair remained scarce. gp130, a type of glycoprotein, functions as a co-receptor for all cytokines in the IL-6 family.

    Article Title: Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress
    Article Snippet: The following antibodies were used: Synapsin-I (SYN, 1:4000, Abclone), Synaptophysin (SYP, 1:4000, Proteintech), Brain-derived neurotrophic factor (BDNF, 1:2000, Abcam), Postsynaptic density protein 95 (PSD95, 1:4000, Proteintech), β-Tubulin (1:10000, HUABIO), p-ERK (1:1000, Cell Signaling Technology), ERK (1:2000, Cell Signaling Technology), p-CREB (1:1000, Cell Signaling Technology), and CREB (1:2000, Cell Signaling Technology).

    Article Title: Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress.
    Article Snippet: Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry.. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as antiviral, antioxidant, and anti-inflammatory effects.. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the antiviral qualities of BBR against SGIV.

    Article Title: Autophagy inhibition potentiates energy restriction-induced cell death in hepatocellular carcinoma cells.
    Article Snippet: Funding information University of Sharjah, Grant/Award Number: 2201110357 Abstract Hepatocellular carcinoma (HCC) significantly contributes to cancer-related mortality due to the limited response of HCC to current anticancer therapies, thereby necessitating more effective treatment approaches.. Energy restriction mimetic agents (ERMAs) have emerged as potential therapies in targeting the Warburg effect, a unique metabolic process in cancer cells.. However, ERMAs exhibit limited efficacy when used as monotherapy.

    Article Title: Neuroprotective effects of the salidroside derivative SHPL-49 via the BDNF/TrkB/Gap43 pathway in rats with cerebral ischemia.
    Article Snippet: Membranes were incubated with 5% nonfat milk (Beyotime) or BSA (Beyotime) followed by overnight incubation at 4 ◦C with the following primary antibodies: BDNF (1:1000, Abcam), TrkB (1:1000, Abclonal), p-AKT (1:1000, Cell Signaling Technology), AKT (1:1000, Cell Signaling Technology), p-ERK (1:1000, Cell Signaling Technology), ERK (1:1000, Cell Signaling Technology), or growthassociated protein 43 (Gap43) (1:200, Santa Cruz).

    Article Title: Marine derivative CHNQD-00603 reverses bone marrow mesenchymal stem cells senescence by enhancing autophagy through the AKT/ERK/mTOR signaling pathway
    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with the primary antibodies GAPDH(1:2000; Elabscience, cat. no. E-AB-20059, China), P62 (1:1000; Catalog No.AF5484; A nity Biosciences), LC3(1:1000; Proteintech, cat. no. 16400-1-AP, China), AKT (1:1000; Cell Signaling Technology, cat. no.C67E7 China), P-AKT(1:1000; Cell Signaling Technology, cat. no.D25E6 China), ERK (1:1000; Cell Signaling Technology, cat. no.137F5 China), P-ERK(1:2000; Cell Signaling Technology, cat. no.D13.14.4E China), overnight at 4 ◦ C and then incubated for 1h at 37°C with secondary antibodies, anti-Rabbit IgG (1:10000, 1:10,000; Proteintech, cat. no. 10285-1-AP).



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    GA effects <t>on</t> <t>ERK1/2</t> phosphorylation in invasive ductal carcinoma-derived KAIMRC1 stem-like cells. Representative western blot analyses showed p-ERK1/2 in KAIMRC1 stem-like cells treated with 100 µg/ml GA for (A) 30 to 120 min and with (B) 50–100 µg/ml GA or unglycated BSA for 90 min of incubation. Bar graphs show the relative expression level of p-ERK1/2 normalized to total ERK1/2. GAPDH served as the loading control. Data are presented as the mean ± SD from three independent experiments. Statistical significance relative to the untreated control is indicated as *P < 0.05 and **P < 0.01. C, control; GA, glycated albumin; p-ERK1/2, phosphorylated-ERK1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
    P Erk, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GA effects <t>on</t> <t>ERK1/2</t> phosphorylation in invasive ductal carcinoma-derived KAIMRC1 stem-like cells. Representative western blot analyses showed p-ERK1/2 in KAIMRC1 stem-like cells treated with 100 µg/ml GA for (A) 30 to 120 min and with (B) 50–100 µg/ml GA or unglycated BSA for 90 min of incubation. Bar graphs show the relative expression level of p-ERK1/2 normalized to total ERK1/2. GAPDH served as the loading control. Data are presented as the mean ± SD from three independent experiments. Statistical significance relative to the untreated control is indicated as *P < 0.05 and **P < 0.01. C, control; GA, glycated albumin; p-ERK1/2, phosphorylated-ERK1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
    Anti P Erk Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GA effects on ERK1/2 phosphorylation in invasive ductal carcinoma-derived KAIMRC1 stem-like cells. Representative western blot analyses showed p-ERK1/2 in KAIMRC1 stem-like cells treated with 100 µg/ml GA for (A) 30 to 120 min and with (B) 50–100 µg/ml GA or unglycated BSA for 90 min of incubation. Bar graphs show the relative expression level of p-ERK1/2 normalized to total ERK1/2. GAPDH served as the loading control. Data are presented as the mean ± SD from three independent experiments. Statistical significance relative to the untreated control is indicated as *P < 0.05 and **P < 0.01. C, control; GA, glycated albumin; p-ERK1/2, phosphorylated-ERK1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

    Journal: Oncology Letters

    Article Title: Methylglyoxal-derived glycated albumin enhances the stemness potential of invasive ductal carcinoma-derived breast cancer stem-like cell line KAIMRC1

    doi: 10.3892/ol.2026.15541

    Figure Lengend Snippet: GA effects on ERK1/2 phosphorylation in invasive ductal carcinoma-derived KAIMRC1 stem-like cells. Representative western blot analyses showed p-ERK1/2 in KAIMRC1 stem-like cells treated with 100 µg/ml GA for (A) 30 to 120 min and with (B) 50–100 µg/ml GA or unglycated BSA for 90 min of incubation. Bar graphs show the relative expression level of p-ERK1/2 normalized to total ERK1/2. GAPDH served as the loading control. Data are presented as the mean ± SD from three independent experiments. Statistical significance relative to the untreated control is indicated as *P < 0.05 and **P < 0.01. C, control; GA, glycated albumin; p-ERK1/2, phosphorylated-ERK1/2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

    Article Snippet: Mouse primary monoclonal antibodies directed against extracellular signal-regulated kinase (ERK)1 (clone G-8; cat. no. sc-271269), phosphorylated-ERK1/2 (p-ERK1/2; clone E-4; Tyr204 of ERK1; cat. no. sc-7383), OCT3/4 (clone A-9; cat. no. sc-365509) and RAGE (clone E-1; cat. no. sc-74473) were obtained from Santa Cruz Biotechnology, Inc.

    Techniques: Phospho-proteomics, Derivative Assay, Western Blot, Incubation, Expressing, Control